Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

“地理素养与测绘技能”双驱动的GIS专业大学生创新能力培养模式的探索     

蔡忠亮  翁敏  苏世亮  亢孟军  费腾  田晶  任福 《测绘通报》2020,(8):148-152

在信息技术快速发展和万众创新、大众创业的时代背景下，创新与创新人才已成为国家发展的重要推动力，创新教育和创新人才培养成为高校的重要任务。本文以武汉大学地理信息科学（GIS）专业为例，结合专业特点和优势，探索了"地理素养、测绘技能"驱动下的GIS专业大学生创新能力的培养模式和"厚基础、重知识、强技能"的贯通式培养途径。围绕创新人才培养和成长的内在因素和外部条件，从人才培养方案与课程体系、创新型教师团队与新型教学方法、教学科研创新平台与创新条件、创新人才发展评价与激励机制等4个方面开展了创新型发展探索。结合实践表明，本模式取得了显著的创新成果，产生了良好的示范辐射作用。  相似文献   

基于熵权TOPSIS模型的海岛地区资源环境承载力研究——以舟山普陀区、定海区为例   总被引：2，自引：0，他引：2  

徐文斌  郭灿文  王晶  程义远  徐鹏杰  张宏晔 《海洋通报》2018,(1):9-16

随着海洋强国、"一带一路"、生态文明建设等国家战略的不断推进,海岛在国家政治、军事、经济、社会、生态中的地位不断提高,海岛开发、利用的规模越来越大,其生态保护也面临着新的挑战。本文依据资源环境承载力内涵,兼顾海岛资源环境特点,研究形成海岛地区资源环境承载力评价指标体系,综合应用熵权法、TOPSIS(逼近理想解排序方法)模型评价了典型海岛地区——普陀区与定海区2009-2015年间的资源环境承载力状况,结果表明:普陀和定海资源环境承载力水平呈稳步提升趋势,各个时期的环境保护政策和节能减排相关措施在环境保护与污染治理等方面起到了较为积极的作用。最后,在基于生态系统的海岛综合管理框架内提出了对策建议。  相似文献   

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes     

Zhang Shicui  Wu Xianhan  Zhou Jing  Wang Renxue  Wu Shangqin 《中国海洋湖沼学报》1990,8(3):263-272

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica  相似文献   

Fluid Inclusion Feature of Gold-Bearing Quartz Veins from the Jinniushan Gold Deposit, Shandong, China     

Shen Ping  Shen Yuanchao  Li Guangming  LIU Tiebing  ZENG Qingdong 《世界地质(英文版)》2002,5(2)

The auriferous veins at Jinniushan occurs within the Jinniushan faulted zone in the Kunyushan Granite. Optical observation reveals that gold ore body formed during the main stage of hydrothermal activity. Detailed geothermometric studies of fluid inclusions from the veins show that the forming temperature ranges between 130℃ and 370℃ and the salinity is from 4.01 to 15.21 wvt percent NaCl. The ore-forming fluid is featured by low to moderate salinity, and low to moderate temperature. According to investigations of the values of vapor/liquid and temperatures of the ore-forming fluids, we propose that the boiling fluid inclusions exist in the main mineralization stages. Fluid boiling is suggested as a mechanism for the precipitation of gold from the hydrothermal fluid in the Jinniushan gold deposit.  相似文献   

小卫星数据的变化监测能力分析     

李静  党福星  李志忠 《测绘科学》2009,34(3)

为了促进小卫星数据在地质灾害监测中的应用与推广,本文以监测采矿图斑变化为目标,通过对北京地区的2006年和2007年两期北京一号小卫星4m全色影像采用正射校正、配准和迭加对比的方法发现变化信息。结合1∶50 000影像图、野外调查和测量,确定变化图斑的边界、变化原因和规模,并对小卫星全色图像的变化监测能力进行分析和总结。本文通过对小卫星4m全色影像数据的分析,基本上可以概括出其变化监测能力。  相似文献   

邻菲啰啉在测定亚铁中的保护效果          下载免费PDF全文

张会堂  刘瑄  刘渝燕  刘晶  巩宝珍 《山东国土资源》2003,19(1):54-56

样品在分解过程中 ,亚铁易被空气部分氧化 ,致使测试结果偏低 ,采用在HF H2 SO4溶矿过程中加入邻菲卟罗啉 ,与亚铁形成络合物 ,对保护亚铁具有较好的效果。拟定了在塑料坩埚中加邻菲卟罗啉、H2 SO4及HF ,中高温电热板溶矿 ,容量法测定样品中的亚铁 ,方法精密度为 0 .2 3% ,样品加标准回收率 98.8%～ 10 1.9%。此方法具有较好的应用效果  相似文献   

Study of a model for correcting the effects of horizontal advection on surface fluxes measurement based on remote sensing     

Tian  Jing  Zhang  Renhua  Sun  Xiaomin  Zhu  Zhilin  Zhou  Yanlian 《中国科学:地球科学(英文版)》2006,49(2):273-280

As well known, the methods of remote sensing and Bowen Ratio for retrieving surface flux are based on energy balance closure; however, in most cases, surface energy observed in experiment is lack of closure. There are two main causes for this: one is from the errors of the observation devices and the differences of their observational scale; the other lies in the effect of horizontal advection on the surface flux measurement. Therefore, it is very important to estimate the effects of horizontal advection quantitatively. Based on the local advection theory and the surface experiment, a model has been proposed for correcting the effect of horizontal advection on surface flux measurement, in which the relationship between the fetch of the measurement and pixel size for remote sensed data was considered. By means of numerical simulations, the sensitivities of the main parameters in the model and the scaling problems of horizontal advection were analyzed. At last, by using the observational data acquired in agricultural field with relatively homogeneous surface, the model was validated.

  相似文献   

Tanlu Fault Zone and Gold Ore Metallogenesis     

SHEN Yuanchao  ZENG Qingdong  LIU Tiebing 《东北亚地学研究》2002,(1)

Tanlu Fault Zone (TFZ) is a large NE trending deep fracture system in East China and is about 2500 km long. It extends from the south of Lujiang to Tancheng, and passes through the Bohai Sea and continues to the north, where it branches into Yilan ?Shulan Fault and Dunhua ?Mishan Fault. TFZ is a long lasting huge deep - rooted lithospherical fault system with different characteristics in different parts and at different periods of its evolution. TFZ also controls the distribution and occur-rences of many superlarge, large, middle and small sized gold ore deposits, e. g. Jiapigou, Haigou, Xiaoxihancha and Ciwei-gou gold deposits in Northeast China; Linglong, Jiaojia, Sanshandao, Taishang, Xincheng, Rushan, and Pengjiakuang gold deposits in Jiaodong Peninsula of Shandong Province; and Guilaizhuang and Yinan in Western Shandong Province.  相似文献   

在BPL信道上增加数据广播内容的探讨   总被引：1，自引：1，他引：0  

吴海涛  边玉敬 《时间频率学报》2001,24(1):38-44

分析了现有罗兰C（包括BPL）在授时方面存在的不足，提出了在BPL信道上实现数据广播的主要目的。详细讨论了各种可能的数据信息的结构，并给出了有关参数和数据的计算方式和公式。认为发播内容的选择是由期望的系统功能决定的，广播内容的编排则与具体实现技术相关。最后给出了在不同调制方法下的传输速率，这是合理编排广播内容的基础。  相似文献